mouse anti neun monoclonal antibody  (Proteintech)

Journal: PeerJ

Article Title: Exercise alleviates allodynia and hyperalgesia concomitant with improvements in aberrant primary afferents and spinal circuit inhibition in the dorsal horn of rats with incomplete spinal cord injury

doi: 10.7717/peerj.20699

Figure Lengend Snippet: (A) Representative immunofluorescence images of co-staining of VGluT1 (green), NeuN (red) and DAPI (blue) in each group ( n = 6). (B) Representative Western blotting images of VGluT1 in each group ( n = 6). (C) The statistical graph of the normalized optical density of VGluT1. (D) The statistical graph of the relative density of VGluT1. Statistical analysis used one-way ANOVA with subsequent Bonferroni post hoc test. Values are expressed as the mean ± SEM. * P < 0.05. Scale bar: 100 µm.

Article Snippet: Then, the sections were permeated in PBS with 0.5% Triton X-100 (#T8200; Solarbio, Beijing, China) for 15 min and blocked in 5% Bovin Serum Albumin (BSA, BSAS 1.0; Bovogen, East Keilor, Australia) for 30 min, followed by incubation with primary antibodies: rabbit anti-GAD65+GAD67 monoclonal antibody (ab183999, division 1:250; Abcam, Waltham, MA, USA), rabbit anti-KCC2 monoclonal antibody (ab259969, division 1:200; Abcam, USA), rabbit anti-VGluT1 monoclonal antibody (ab227805, division 1:100; Abcam, USA), mouse anti-NeuN monoclonal antibody (#94403, division 1:400; Cell Signaling Technology, Danvers, MA, USA), or mouse anti-CGRP monoclonal antibody (ab81887, division 1:100; Abcam) diluted in 5% BSA overnight at 4 °C.

Techniques: Immunofluorescence, Staining, Western Blot

Journal: PeerJ

Article Title: Exercise alleviates allodynia and hyperalgesia concomitant with improvements in aberrant primary afferents and spinal circuit inhibition in the dorsal horn of rats with incomplete spinal cord injury

doi: 10.7717/peerj.20699

Figure Lengend Snippet: (A) Representative immunofluorescence images of co-staining of GAD65+67 (green), NeuN (red) and DAPI (blue) in each group ( n = 6). (B) Representative Western blotting images of GAD65+67 in each group ( n = 6). (C) The statistical graph of the normalized optical density of GAD65+67. (D) The statistical graph of the relative density of GAD65+67. Statistical analysis used one-way ANOVA with subsequent Bonferroni post hoc test. Values are expressed as the mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. Scale bar: 100 µm.

Article Snippet: Then, the sections were permeated in PBS with 0.5% Triton X-100 (#T8200; Solarbio, Beijing, China) for 15 min and blocked in 5% Bovin Serum Albumin (BSA, BSAS 1.0; Bovogen, East Keilor, Australia) for 30 min, followed by incubation with primary antibodies: rabbit anti-GAD65+GAD67 monoclonal antibody (ab183999, division 1:250; Abcam, Waltham, MA, USA), rabbit anti-KCC2 monoclonal antibody (ab259969, division 1:200; Abcam, USA), rabbit anti-VGluT1 monoclonal antibody (ab227805, division 1:100; Abcam, USA), mouse anti-NeuN monoclonal antibody (#94403, division 1:400; Cell Signaling Technology, Danvers, MA, USA), or mouse anti-CGRP monoclonal antibody (ab81887, division 1:100; Abcam) diluted in 5% BSA overnight at 4 °C.

Techniques: Immunofluorescence, Staining, Western Blot

Journal: PeerJ

Article Title: Exercise alleviates allodynia and hyperalgesia concomitant with improvements in aberrant primary afferents and spinal circuit inhibition in the dorsal horn of rats with incomplete spinal cord injury

doi: 10.7717/peerj.20699

Figure Lengend Snippet: (A) Representative immunofluorescence images of co-staining of KCC2 (green), NeuN (red) and DAPI (blue) in each group ( n = 6). (B) Representative Western blotting images of KCC2 in each group ( n = 6). (C) The statistical graph of the normalized optical density of KCC2. (D) The statistical graph of the relative density of KCC2. Statistical analysis used one-way ANOVA with subsequent Bonferroni post hoc test. Values are expressed as the mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. Scale bar: 100 µm.

Article Snippet: Then, the sections were permeated in PBS with 0.5% Triton X-100 (#T8200; Solarbio, Beijing, China) for 15 min and blocked in 5% Bovin Serum Albumin (BSA, BSAS 1.0; Bovogen, East Keilor, Australia) for 30 min, followed by incubation with primary antibodies: rabbit anti-GAD65+GAD67 monoclonal antibody (ab183999, division 1:250; Abcam, Waltham, MA, USA), rabbit anti-KCC2 monoclonal antibody (ab259969, division 1:200; Abcam, USA), rabbit anti-VGluT1 monoclonal antibody (ab227805, division 1:100; Abcam, USA), mouse anti-NeuN monoclonal antibody (#94403, division 1:400; Cell Signaling Technology, Danvers, MA, USA), or mouse anti-CGRP monoclonal antibody (ab81887, division 1:100; Abcam) diluted in 5% BSA overnight at 4 °C.

Techniques: Immunofluorescence, Staining, Western Blot

Journal: BMC Pharmacology & Toxicology

Article Title: Vitamin E-valproate co-therapy attenuated oxidative stress, neuroinflammation, related cognitive deficits and neuronal damage in Cypermethrin exacerbated seizure

doi: 10.1186/s40360-025-01027-6

Figure Lengend Snippet: Representative photomicrographs of NeuN + neurons in the CA1 ( A ), CA3 ( B ), DG ( C ) and Parvalbumin + interneurons in the CA1 ( D ), CA3 ( E ), ( F ); and the respective graphs of the estimated immunoreactive cells in rats exposed to corn-oil (CTR), Pentylenetetrazol (PTZ), Cypermethrin (CPM) and PTZ plus CPM respectively. Asterisks (*, **, ***, ****) indicate statistically significant differences at P = 0.05, One-Way ANOVA

Article Snippet: The sections were then incubated for 2 h at room temperature in primary antibodies: Goat polyclonal Iba-1 (Abcam, USA, ab5076) at 1:250, Mouse monoclonal NeuN-HRP conjugated (Santa Cruz, USA, 2E1: sc-33673) at 1:150 or Rabbit polyclonal PV antibody (Novus Biologicals, USA, NB120-11427) at 1:1000, followed by 5 min wash in PBS.

Techniques:

Journal: BMC Pharmacology & Toxicology

Article Title: Vitamin E-valproate co-therapy attenuated oxidative stress, neuroinflammation, related cognitive deficits and neuronal damage in Cypermethrin exacerbated seizure

doi: 10.1186/s40360-025-01027-6

Figure Lengend Snippet: Representative photomicrographs of NeuN + neurons in the CA1 ( A ), CA3 ( B ), DG ( C ) and Parvalbumin + interneurons in the CA1 ( D ), CA3 ( E ), ( F ); and the respective graphs of the estimated immunoreactive cells in rats exposed to Pentylenetetrazol and Cypermethrin without intervention (CPM + PTZ), and with interventions, Valproic Acid (VAP), Vitamin E (VIT E) and the combination. Plus (+) and Minus (-) signs indicate presence and absence respectively. Asterisks (*, **, ***, ****) indicate statistically significant differences at P = 0.05, One-Way ANOVA

Article Snippet: The sections were then incubated for 2 h at room temperature in primary antibodies: Goat polyclonal Iba-1 (Abcam, USA, ab5076) at 1:250, Mouse monoclonal NeuN-HRP conjugated (Santa Cruz, USA, 2E1: sc-33673) at 1:150 or Rabbit polyclonal PV antibody (Novus Biologicals, USA, NB120-11427) at 1:1000, followed by 5 min wash in PBS.

Techniques: